Difference between revisions of "Part:BBa K4789008"
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Exons of mitogen-activated protein kinase kinase kinase 7 (MAP3K7) was skipped in MDS patients. We have constructed the sensor based on the exon skipping of MAP3K7 to the plasmid containing reporter genes, which monitor the alteration of RNA splicing in cells. | Exons of mitogen-activated protein kinase kinase kinase 7 (MAP3K7) was skipped in MDS patients. We have constructed the sensor based on the exon skipping of MAP3K7 to the plasmid containing reporter genes, which monitor the alteration of RNA splicing in cells. | ||
The Branch point site of introns play a key role in the alternative splicing of RNA | The Branch point site of introns play a key role in the alternative splicing of RNA | ||
| − | Now we want to improve RNA splicing efficiency, we mutate the branch point site " | + | Now we want to improve RNA splicing efficiency, we mutate the branch point site "atctgAg" to "atctgAc". |
Revision as of 08:18, 30 September 2023
GTGAGT-recessive exon-CCACAG of MAP3K7 (improved)
Our improved experiment is based upon the YiYe-China iGEM project from 2022(https://2022.igem.wiki/yiye-china/,BBa_K4789008). Exons of mitogen-activated protein kinase kinase kinase 7 (MAP3K7) was skipped in MDS patients. We have constructed the sensor based on the exon skipping of MAP3K7 to the plasmid containing reporter genes, which monitor the alteration of RNA splicing in cells. The Branch point site of introns play a key role in the alternative splicing of RNA Now we want to improve RNA splicing efficiency, we mutate the branch point site "atctgAg" to "atctgAc".
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]