Difference between revisions of "Part:BBa K4789007"

Latest revision as of 07:54, 30 September 2023

pcDNA-pre-miR-145

Usage and Biology

MicroRNAs (miRNAs), a novelty-defined class of regulatory genes, have revolutionized principles of classical bimolecular. These RNAs regulate the expression of a gene through inhibition of translational initiation or targeting mRNAs for degradation. MiR-145 has the diagnose value in patients with cervical cancer[1].Our team also focus on the cervical cancer screening. Previous researches have discovered miR-145 could inhibit cervical cancer progression through targeting WNT2B by Wnt/β-catenin pathway[2] or miR-145/RCAN3 axis[3] and so on. LncRNAs work as ‘miRNA sponges’ through binding to specific miRNAs. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), long non-coding RNA, has been reported to be upregulated in cervical cancer tissues and promote cancer cell invasion and metastasis[4]. Pre-mir-145 could bind to the sequence of MALAT1 according to the prediction database miRcode(Figure 1)[5]. This mechanism gives rise to our idea about fusing a sponge RNA based on the sequences of lncRNA with binding sites complementary to the sequence of miRNA into a plasmid contained the reporter gene. In our study, we cloned the pre-miR145 sequence into the basic part pcDNA 3.0 plasmid. Then the miR22 could overexpressed in the Hela cell.

            
                  Figure 1   miR-145and LncRNA binding site

Reference

[1]Yu Furong,Liu Jie,Dong Weilei et al. The diagnostic value of miR-145 and miR-205 in patients with cervical cancer.[J] .Am J Transl Res, 2021, 13: 1825-1832.

[2 ]Li Qi,Yu Xiaohong,Yang Liangliang,MiR-145 inhibits cervical cancer progression and metastasis by targeting WNT2B by Wnt/β-catenin pathway.[J] .Int J Clin Exp Pathol, 2019, 12: 3740-3751.

[3]Zhang Xian-Yu,Ma Huan,Li Jing et al. Functional implications of miR-145/RCAN3 axis in the progression of cervical cancer.[J] .Reprod Biol, 2020, 20: 140-146.

[4]Han Xiting,Wang Qian,Wang Yan et al. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1/microRNA-202-3p/periostin axis modulates invasion and epithelial-mesenchymal transition in human cervical cancer.[J] .J Cell Physiol, 2019, 234: 14170-14180.

[5] http://www.mircode.org/

Sequence and Features