Difference between revisions of "Part:BBa K4765001"
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The protein attached to the C-terminus of intimin can achieve outer membrane localization. In our project, intimin is fused with antigens, nanobodies, and lectins, facilitating their display on the membrane of Escherichia coli. This approach allows for the binding of E. coli- E. coli and E. coli to cyanobacteria. | The protein attached to the C-terminus of intimin can achieve outer membrane localization. In our project, intimin is fused with antigens, nanobodies, and lectins, facilitating their display on the membrane of Escherichia coli. This approach allows for the binding of E. coli- E. coli and E. coli to cyanobacteria. | ||
===Characterization=== | ===Characterization=== | ||
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| + | <!-- --> | ||
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K4765001 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K4765001 parameters</partinfo> | ||
| + | <!-- --> | ||
==Reference== | ==Reference== | ||
Revision as of 04:44, 30 September 2023
intimin
Introduction
Intimin is a surface display system ,it was first described by Piñero-Lambea et. Al[1]. and tested by iGEM22_GreatBay_SCIE. It includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a 12-stranded beta-barrel for transmembrane insertion.
Usage and Biology
The protein attached to the C-terminus of intimin can achieve outer membrane localization. In our project, intimin is fused with antigens, nanobodies, and lectins, facilitating their display on the membrane of Escherichia coli. This approach allows for the binding of E. coli- E. coli and E. coli to cyanobacteria.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1244
Reference
- ↑ Piñero-Lambea, C., Bodelón, G., Fernández-Periáñez, R., Cuesta, A. M., Álvarez-Vallina, L., & Fernández, L. Á. (2015). Programming controlled adhesion of E. coli to target surfaces, cells, and tumors with synthetic adhesins. ACS Synthetic Biology, 4(4), 463–473. https://doi.org/10.1021/sb500252a