Difference between revisions of "Part:BBa K4789009"
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− | 1 | + | The introduction of our experiment |
+ | Our improved experiment is based upon the YiYe-China iGEM project from 2022(https://2022.igem.wiki/yiye-china/, https://parts.igem.org/Part:BBa_K4218004). The project aims to provide a plasmid sensor to detect intracellular alternative splicing for early diagnosis of myelodysplastic syndromes(MDS). The project had established a dual luciferase reporter system to monitor the alteration of RNA splicing in cells These reporters incorporate segments of both the intronic and exonic sequences of MAP3K7 or ZNF91 within the luciferase gene (Fig 1). The activity of luciferase can be used to measure the changes of intracellular splicing, so as to play an auxiliary role in the diagnosis of MDS. | ||
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Revision as of 04:22, 30 September 2023
The MAP3K7-LUC improved plasmids
The introduction of our experiment Our improved experiment is based upon the YiYe-China iGEM project from 2022(https://2022.igem.wiki/yiye-china/, https://parts.igem.org/Part:BBa_K4218004). The project aims to provide a plasmid sensor to detect intracellular alternative splicing for early diagnosis of myelodysplastic syndromes(MDS). The project had established a dual luciferase reporter system to monitor the alteration of RNA splicing in cells These reporters incorporate segments of both the intronic and exonic sequences of MAP3K7 or ZNF91 within the luciferase gene (Fig 1). The activity of luciferase can be used to measure the changes of intracellular splicing, so as to play an auxiliary role in the diagnosis of MDS.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2459
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3661