Difference between revisions of "Part:BBa K4586029"
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<partinfo>BBa_K4586029 short</partinfo> | <partinfo>BBa_K4586029 short</partinfo> | ||
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| + | == Usage and Description == | ||
This composite part codes for our tissue-specific switch and consists of two elements: first, a stop codon (UAG) sensor that is designed to be complementary to a specific RNA within the target cell or tissue; in our case, our target is the auto-reactive B-cells that secrete ACPA, and the sensor is complementary to the variable chain of ACPA mRNA; second, a two-hairpin structure flanking the sensor, which is called MS2, that has binding affinity to MCP and is conjugated to the ADAR2 catalytic portion in order to enhance the sensitivity and amplify the signal of our therapeutic cargo (Cas12k). | This composite part codes for our tissue-specific switch and consists of two elements: first, a stop codon (UAG) sensor that is designed to be complementary to a specific RNA within the target cell or tissue; in our case, our target is the auto-reactive B-cells that secrete ACPA, and the sensor is complementary to the variable chain of ACPA mRNA; second, a two-hairpin structure flanking the sensor, which is called MS2, that has binding affinity to MCP and is conjugated to the ADAR2 catalytic portion in order to enhance the sensitivity and amplify the signal of our therapeutic cargo (Cas12k). | ||
Revision as of 13:09, 29 September 2023
DART-V-ADAR (tissue specific switch)
Usage and Description
This composite part codes for our tissue-specific switch and consists of two elements: first, a stop codon (UAG) sensor that is designed to be complementary to a specific RNA within the target cell or tissue; in our case, our target is the auto-reactive B-cells that secrete ACPA, and the sensor is complementary to the variable chain of ACPA mRNA; second, a two-hairpin structure flanking the sensor, which is called MS2, that has binding affinity to MCP and is conjugated to the ADAR2 catalytic portion in order to enhance the sensitivity and amplify the signal of our therapeutic cargo (Cas12k).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]