Difference between revisions of "Part:BBa K4650004"
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| − | + | BBa_K4650004: contained BBa_K4650000+BBa_K4650001 (pGal1,10-CBD) and was transformed into yeast wild-type,BY4741, to make the BY4741 containing BBa_K4650004 used as a control in our project. The CBM1 in S. Pombe encodes for the cellulose binding domain (CBD) protein cloned downstream of the galactose promoter, an inducible promoter to manipulate the transcription of CBD in the presence of galactose. The purpose of manipulating CBD was used as a carrier for our team’s spider silk proteins to scoby membranes in the composite parts, BBa_K4650005, and BBa_K4650006. | |
Revision as of 08:44, 29 September 2023
BBa_K4650004: contained BBa_K4650000+BBa_K4650001 (pGal1,10-CBD) and was transformed into yeast wild-type,BY4741, to make the BY4741 containing BBa_K4650004 used as a control in our project. The CBM1 in S. Pombe encodes for the cellulose binding domain (CBD) protein cloned downstream of the galactose promoter, an inducible promoter to manipulate the transcription of CBD in the presence of galactose. The purpose of manipulating CBD was used as a carrier for our team’s spider silk proteins to scoby membranes in the composite parts, BBa_K4650005, and BBa_K4650006.