Difference between revisions of "Part:BBa K4579000"
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This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTet promoter upstream of a hammerhead ribozyme (HHRz), the latter of which helps insulate the transcript from sequence-specific interference effects. The pTet promoter contains two TetO operators that can be bound by the repressor protein TetR, causing downregulation of transcription. TetR may be removed from the TetO sites when bound by the inducer molecule anhydrotetracycline (aTc), allowing for the selective induction of transcription in cells containing both pTet and the tetR gene. | This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTet promoter upstream of a hammerhead ribozyme (HHRz), the latter of which helps insulate the transcript from sequence-specific interference effects. The pTet promoter contains two TetO operators that can be bound by the repressor protein TetR, causing downregulation of transcription. TetR may be removed from the TetO sites when bound by the inducer molecule anhydrotetracycline (aTc), allowing for the selective induction of transcription in cells containing both pTet and the tetR gene. | ||
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Revision as of 22:18, 28 September 2023
PTet* promoter + RBS
This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTet promoter upstream of a hammerhead ribozyme (HHRz), the latter of which helps insulate the transcript from sequence-specific interference effects. The pTet promoter contains two TetO operators that can be bound by the repressor protein TetR, causing downregulation of transcription. TetR may be removed from the TetO sites when bound by the inducer molecule anhydrotetracycline (aTc), allowing for the selective induction of transcription in cells containing both pTet and the tetR gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]