Difference between revisions of "Part:BBa K4814011"

 
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<partinfo>BBa_K4814011 short</partinfo>
 
<partinfo>BBa_K4814011 short</partinfo>
  
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===Usage and Biology===
 
===Usage and Biology===
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We have used the codon optimized version of mCherry by Shaner, N. et al (2004), derived from the Discosoma (DsRed). This protein is used to construct a FRET pair to detect DNA damage in mammalian cells. Please refer to BBa_K4814006.
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Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567–1572. https://doi.org/10.1038/nbt1037
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Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. https://doi.org/10.1073/pnas.082243699
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Revision as of 13:51, 28 September 2023


Mammalian Codon Optimized mCherry


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]