Difference between revisions of "Part:BBa K4727104"
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<partinfo>BBa_K4727104 short</partinfo> | <partinfo>BBa_K4727104 short</partinfo> | ||
| − | This guide DNA has been designed to work with dCas9 to target AMR related genes in | + | This guide DNA has been designed to work with dCas9 to target AMR related genes in A. baumannii. The target is positioned 56bp from the starting codon |
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| + | blaADC, also called blaAmpC, is a class C β-lactamase, i.e. a cephalosporinase encoded by the chromosome in A. baumannii. It involves resistance to Ampicillin, especially when it is overexpressed due to the insertion of short transposon sequences, which are stronger promoters than the intrinsic one. | ||
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Revision as of 12:04, 27 September 2023
sgDNA for AmpC 56 for A. baumannii
This guide DNA has been designed to work with dCas9 to target AMR related genes in A. baumannii. The target is positioned 56bp from the starting codon
blaADC, also called blaAmpC, is a class C β-lactamase, i.e. a cephalosporinase encoded by the chromosome in A. baumannii. It involves resistance to Ampicillin, especially when it is overexpressed due to the insertion of short transposon sequences, which are stronger promoters than the intrinsic one.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]