Difference between revisions of "Part:BBa K258001"
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Lard 1 was designed for the secretion of fusion proteins.The LARD 1 included four glycine-rich repeats comprising a β-roll structure, and were added to the C-terminus of test proteins. Either Pro-Gly linker or Factor Xa site can be added between fusion proteins and LARD 1. Upon supplementation of E. coli with ABC transporter from E. chrysanthemi (ABC transporter PrtDEF), which function well in the phylogenetically-neighboring genus E.coli, EGF-LARDs were excreted into the culture supernatant. For LARD1, linkers may be engineered to | Lard 1 was designed for the secretion of fusion proteins.The LARD 1 included four glycine-rich repeats comprising a β-roll structure, and were added to the C-terminus of test proteins. Either Pro-Gly linker or Factor Xa site can be added between fusion proteins and LARD 1. Upon supplementation of E. coli with ABC transporter from E. chrysanthemi (ABC transporter PrtDEF), which function well in the phylogenetically-neighboring genus E.coli, EGF-LARDs were excreted into the culture supernatant. For LARD1, linkers may be engineered to | ||
have a Factor Xa cleavage site. LARD1 also contained residues | have a Factor Xa cleavage site. LARD1 also contained residues | ||
| − | 303–476 of TliA. | + | 303–476 of TliA. The thermostable lipase, TliA, from which Jung Hoon Ahn |
| + | designed LARD1, has four GGXGXD consensus sequences | ||
| + | and EGVLIS as its final six C-terminal residues, showing | ||
| + | similar organization of several hydrophobic residues preceded | ||
| + | by an acidic residue, Glu. | ||
<html> | <html> | ||
Revision as of 10:58, 17 October 2009
Lipase ABC transporter recognition domain (LARD 1)
Lard 1 was designed for the secretion of fusion proteins.The LARD 1 included four glycine-rich repeats comprising a β-roll structure, and were added to the C-terminus of test proteins. Either Pro-Gly linker or Factor Xa site can be added between fusion proteins and LARD 1. Upon supplementation of E. coli with ABC transporter from E. chrysanthemi (ABC transporter PrtDEF), which function well in the phylogenetically-neighboring genus E.coli, EGF-LARDs were excreted into the culture supernatant. For LARD1, linkers may be engineered to have a Factor Xa cleavage site. LARD1 also contained residues 303–476 of TliA. The thermostable lipase, TliA, from which Jung Hoon Ahn designed LARD1, has four GGXGXD consensus sequences and EGVLIS as its final six C-terminal residues, showing similar organization of several hydrophobic residues preceded by an acidic residue, Glu.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]