Difference between revisions of "Part:BBa K4650000"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4650000 short</partinfo>
 
<partinfo>BBa_K4650000 short</partinfo>
  
−
Gal promoter is in the plasmid and genes are cloned downstream of the pGal promoter, so the cloned genes can be induced in the presence of galactose. The galactose promoter can be induced in the presence of galactose and suppressed in the presence of glucose or raffinose.  
+
The purpose of using the pGal 1,10 promoter is to regulate gene expression. In the presence of galactose, it induces downstream of the gene’s mRNA induction and in the absence of galactose, the downstream of the gene's mRNA induction is off.
 +
 
 
Citation: Investigations in Molecular Cell Biology (O'Connor); 13.1: Regulation of the GAL1 promoter; <https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_
 
Citation: Investigations in Molecular Cell Biology (O'Connor); 13.1: Regulation of the GAL1 promoter; <https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_
  

Latest revision as of 01:02, 27 September 2023


pGal ppromoter

The purpose of using the pGal 1,10 promoter is to regulate gene expression. In the presence of galactose, it induces downstream of the gene’s mRNA induction and in the absence of galactose, the downstream of the gene's mRNA induction is off.

Citation: Investigations in Molecular Cell Biology (O'Connor); 13.1: Regulation of the GAL1 promoter; <https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]