Difference between revisions of "Part:BBa K4905019"
Line 11: Line 11:
 
</body>
 
</body>
 
</html>  
 
</html>  
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><h1>Sequence and Features</h1></span>
 
<partinfo>BBa_K4905019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4905019 SequenceAndFeatures</partinfo>
  
Line 17: Line 17:
 
<body>
 
<body>
 
<h1>References</h1>
 
<h1>References</h1>
 +
<p>
 
[1] Inokuchi K, Mutoh N, Matsuyama S, Mizushima S (1982) Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res 10:6957-6968  
 
[1] Inokuchi K, Mutoh N, Matsuyama S, Mizushima S (1982) Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res 10:6957-6968  
  
Line 22: Line 23:
  
 
[3] Yamamoto, T, Okawa N.,Endo T., and Kaji A. (1991) Expression of chimeric ras protein with OmpF signal peptide in Escherichia coli: localization of OmpF fusion protein in the inner membrane. Appl Microbiol BiotechnoZ 35:615-621  
 
[3] Yamamoto, T, Okawa N.,Endo T., and Kaji A. (1991) Expression of chimeric ras protein with OmpF signal peptide in Escherichia coli: localization of OmpF fusion protein in the inner membrane. Appl Microbiol BiotechnoZ 35:615-621  
−
 
+
</p>
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 09:31, 26 September 2023


The Ompf sequence is a signaling peptide allowing for translocation of a protein to the periplasm

The E.coli K-12 strain possesses a protein, called the outer membrane protein F (Ompf). The precursor of this protein contains a signaling peptide 22 amino acids in length, which is the Ompf signaling sequence[1]. This sequence is used for translocating proteins from the cytoplasm to the periplasm[2]. For this reason, the Ompf sequence can be fused to other proteins for the secretion into the periplasm[3].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Inokuchi K, Mutoh N, Matsuyama S, Mizushima S (1982) Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res 10:6957-6968 [2] Vlasuk GP, Inouye S, Ito H, Itakura K, Inouye M (1983) Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli. J Biol Chem 258:7141-7148 [3] Yamamoto, T, Okawa N.,Endo T., and Kaji A. (1991) Expression of chimeric ras protein with OmpF signal peptide in Escherichia coli: localization of OmpF fusion protein in the inner membrane. Appl Microbiol BiotechnoZ 35:615-621