Difference between revisions of "Part:BBa K4606010:Design"
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===Source=== | ===Source=== | ||
| − | + | Plasmids were obtained from Escherichia coli. | |
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===References=== | ===References=== | ||
Latest revision as of 02:16, 23 September 2023
Vector-ZsGreen-miR-3074-5p-AmpR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin. ZsGreen protein is spontaneously expressed and emits green fluorescence after successful transfection, so we can observe the transfection efficiency of the vector.
Source
Plasmids were obtained from Escherichia coli.