Difference between revisions of "Part:BBa K4579000"

Revision as of 17:12, 22 September 2023

P_Tet* promoter + RBS

This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTet promoter upstream of a hammerhead ribozyme (HHRz), the latter of which helps insulate the transcript from sequence-specific interference effects. The pTet promoter contains two TetO operators that can be bound by the repressor protein TetR, causing downregulation of transcription. TetR may be removed from the TetO sites when bound by the inducer molecule anhydrotetracycline (aTc), allowing for the selective induction of transcription in cells containing both pTet and the tetR gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4579000 short</partinfo>
-This is an inducible promoter part compatible with Golden Gate Assembly. This part can undergo BsaI digestion to produce a linear part with overhangs consistent with the Bee Toolkit syntax (Leonard et al., 2018).  ontains tetO operators that are repressed in the presence of tetR repressor protein. tetR can be removed from the promoter in the presence of anhydrotetracycline (aTc), allowing for the activation of transcription in cells containing both pTet and tetR. In strains only containing pTet, this promoter acts as in a functionally constitutive manner.
+This is an inducible promoter part compatible with Golden Gate Assembly using the Bee Toolkit (BTK) syntax (Leonard et al., 2018). This part undergoes BsaI digestion to produce a linear part with overhangs characteristic of a Type 2 part in the BTK syntax. This part consists of the pTet promoter upstream of a hammerhead ribozyme (HHRz), the latter of which helps insulate the transcript from sequence-specific interference effects. The pTet promoter contains two TetO operators that can be bound by the repressor protein TetR, causing downregulation of transcription. TetR may be removed from the TetO sites when bound by the inducer molecule anhydrotetracycline (aTc), allowing for the selective induction of transcription in cells containing both pTet and the tetR gene.
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