Difference between revisions of "Part:BBa K4656004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | The Plee promotor is under the control of PchA, we designed and compared Ppcha-pcha-Plee-egfp and Ppcha-pcha-egfp circuit. | ||
+ | ===Experiment results=== | ||
+ | We designed two gene fragments as mentinoned: one directly attaching EGFP downstream of the pchA promoter, and the other appending EGFP after the existing gene segment in enterohemorrhagic E. coli. We will compare the responsiveness and feedback levels of these two fragments to butyrate. | ||
+ | <html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/part-plee.jpg"width="466" height="223"></center></html> | ||
===Reference=== | ===Reference=== |
Revision as of 12:56, 22 September 2023
PLEE1
PLEE1 is the promoter of the locus of enterocyte effacement which binds to pchA.
Since the specific binding site of pchA and PLEE1 is still unknown, at present, researchers have purchased Escherichia coli genome from atcc and amplified PLEE1 with different fragments by PCR as candidates, and constructed 6 different kinds of plasmids. +1 is the transcription start site TSS.
"Additionally, the best performing plasmid, pC4S-P4, contains a small part of the ler gene in the PLEE1 promoter region."[1] {The pC4S-P4: pDMB,PpchA-pchA-PLEE1(-98+218bp)-gfp;CmR}
Usage and Biology
The Plee promotor is under the control of PchA, we designed and compared Ppcha-pcha-Plee-egfp and Ppcha-pcha-egfp circuit.
Experiment results
We designed two gene fragments as mentinoned: one directly attaching EGFP downstream of the pchA promoter, and the other appending EGFP after the existing gene segment in enterohemorrhagic E. coli. We will compare the responsiveness and feedback levels of these two fragments to butyrate.
Reference
[1]. Schwan W. R. (2011). Regulation of fim genes in uropathogenic Escherichia coli. World journal of clinical infectious diseases, 1(1), 17–25. https://doi.org/10.5495/wjcid.v1.i1.17.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]