Difference between revisions of "Part:BBa K4656005"

Revision as of 12:41, 22 September 2023

PpchA

PpchA, the promoter of pchA, is present in the butyrate sensor derived from EHEC strain O157:H7, which was discovered by Nakanishi et al. (2009).[1] In the butyrate sensor, PpchA binds to a complex formed by leucine response regulatory protein (Lrp) and butyrate to activate the transcription of pchA regulators.

Reference

[1]. Nakanishi, N., Tashiro, K., Kuhara, S., Hayashi, T., Sugimoto, N., & Tobe, T. (2009). Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. Microbiology (Reading, England), 155(Pt 2), 521–530. https://doi.org/10.1099/mic.0.023499-0

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 143
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 75

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 __NOTOC__
 <partinfo>BBa_K4656005 short</partinfo>
-<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/part-pcha.jpg"width="200" height="190"></center></html>
+<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/part-pcha.jpg"width="220" height="170"></center></html>
 PpchA, the promoter of pchA, is present in the butyrate sensor derived from EHEC strain O157:H7, which was discovered by Nakanishi et al. (2009).[1] In the butyrate sensor, PpchA binds to a complex formed by leucine response regulatory protein (Lrp) and butyrate to activate the transcription of pchA regulators.