Difference between revisions of "Part:BBa K190027:Design"
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===Source=== | ===Source=== | ||
| − | ArsR was obtained from the E. coli K-12 DH10B genome. | + | ArsR was obtained from the ''E. coli'' K-12 DH10B genome. |
MBP was obtained from vector pRE-MBP (kindly provided by Guus Erkens, Membrane Enzymology group, University of Groningen). | MBP was obtained from vector pRE-MBP (kindly provided by Guus Erkens, Membrane Enzymology group, University of Groningen). | ||
===References=== | ===References=== | ||
Revision as of 20:48, 16 October 2009
MBP-ArsR fusion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 360
Illegal BglII site found at 1271 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 58
Illegal SapI.rc site found at 1102
Design Notes
Adding tobacco etch virus (TEV) cleavage site and linker site to facilitate cloning. Adding a SacI restriction site to facilitate non-expensive cloning.
Source
ArsR was obtained from the E. coli K-12 DH10B genome. MBP was obtained from vector pRE-MBP (kindly provided by Guus Erkens, Membrane Enzymology group, University of Groningen).