Difference between revisions of "Part:BBa K190027:Design"

 
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<partinfo>BBa_K190027 short</partinfo>
 
<partinfo>BBa_K190027 short</partinfo>
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===Source===
 
===Source===
  
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ArsR was obtained from the E. coli K-12 DH10B genome
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ArsR was obtained from the E. coli K-12 DH10B genome.
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MBP was obtained from a plasmid
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MBP was obtained from vector pRE-MBP (kindly provided by Guus Erkens, Membrane Enzymology group, University of Groningen).
  
 
===References===
 
===References===

Revision as of 20:48, 16 October 2009

MBP-ArsR fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
    Illegal BglII site found at 1271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal SapI.rc site found at 1102


Design Notes

Adding tobacco etch virus (TEV) cleavage site and linker site to facilitate cloning. Adding a SacI restriction site to facilitate non-expensive cloning.


Source

ArsR was obtained from the E. coli K-12 DH10B genome. MBP was obtained from vector pRE-MBP (kindly provided by Guus Erkens, Membrane Enzymology group, University of Groningen).

References