Difference between revisions of "Part:BBa K4579000"
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<partinfo>BBa_K4579000 short</partinfo> | <partinfo>BBa_K4579000 short</partinfo> | ||
| − | + | This is an inducible promoter part compatible with Golden Gate Assembly. This part can undergo BsaI digestion to produce a linear part with overhangs consistent with the Bee Toolkit syntax (Leonard et al., 2018). ontains tetO operators that are repressed in the presence of tetR repressor protein. tetR can be removed from the promoter in the presence of anhydrotetracycline (aTc), allowing for the activation of transcription in cells containing both pTet and tetR. In strains only containing pTet, this promoter acts as in a functionally constitutive manner. | |
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Revision as of 03:21, 22 September 2023
PTet* promoter + RBS
This is an inducible promoter part compatible with Golden Gate Assembly. This part can undergo BsaI digestion to produce a linear part with overhangs consistent with the Bee Toolkit syntax (Leonard et al., 2018). ontains tetO operators that are repressed in the presence of tetR repressor protein. tetR can be removed from the promoter in the presence of anhydrotetracycline (aTc), allowing for the activation of transcription in cells containing both pTet and tetR. In strains only containing pTet, this promoter acts as in a functionally constitutive manner.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]