Difference between revisions of "Part:BBa K4586019"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4586019 short</partinfo>
 
<partinfo>BBa_K4586019 short</partinfo>
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==Part description==  
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==Part Description==  
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Nuclear localization signals are  short peptides that are responsible for the transport of nuclear protein from the cytoplasm to the nucleus to perform its function.  
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This part codes for the poly-Glycine-Serine (G4S) linker peptide that is a type of flexible,unstructured synthetic peptide linker sequence.
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G4S linker is composed of a core pentapeptide Gly-Gly-Gly-Gly-Ser that is repeated and usually found within scFV-based synthetic receptors as CARs and syn notch receptors.
  
 
==Usage==
 
==Usage==

Revision as of 10:11, 21 September 2023


G4S linker

Part Description

This part codes for the poly-Glycine-Serine (G4S) linker peptide that is a type of flexible,unstructured synthetic peptide linker sequence. G4S linker is composed of a core pentapeptide Gly-Gly-Gly-Gly-Ser that is repeated and usually found within scFV-based synthetic receptors as CARs and syn notch receptors.

Usage

We used this basic part in our design to mediate the transfer of our transcription module VP64 into the nucleus to carry out its action on the target promoter that regulates the expression of our therapeutic agent (Cass12k).




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]