Difference between revisions of "Part:BBa K4586003"

Revision as of 09:54, 21 September 2023

ZF21.16 minCMV Promoter

Part Description

This part codes for conditional promoter its activity based on VP64 transcription factor This promoter is designed to contain multiple repeats recognized by the zinc finger protein 21.16 to direct VP64 activity toward this promoter.it has a high level of transcriptional activity, and it is demonstrated that ZF21 encourages focal adhesion turnover and ECM breakdown at the invadopodia, which suppresses tumor growth.

Usage

This conditioned promoter is implemented in our design as it has a high level of transcriptional activity and is regulated by the VP64 released from the internal domain of our syn notch receptor. The ZF21.16 promoter is used in our design to control the expression of our cargo circuit or our therapeutic agent, which contains (switch + Cas12/gBAFF-R), which has a high level of transcriptional activity and is regulated by the VP64 released from the internal domain of our syn notch receptor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 ==Part Description==
-This part codes for conditional promoter and has activity based on VP64 transcription factor
+This part codes for conditional promoter its activity based on VP64 transcription factor
 This promoter is designed to contain multiple repeats recognized by the zinc finger protein 21.16 to direct VP64 activity toward this promoter.it has a high level of transcriptional activity, and it is demonstrated that ZF21 encourages focal adhesion turnover and ECM breakdown at the invadopodia, which suppresses tumor growth.