Difference between revisions of "Part:BBa K4806222"

 
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<partinfo>BBa_K4806222 short</partinfo>
 
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4806222 SequenceAndFeatures</partinfo>
 
  
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<p>This composite part contains the AβSAP(i)-promotor (<a href=" https://parts.igem.org/Part:BBa_K4806013">BBa_K4806013</a>), the coding sequence of CYP9Q3 (<a href=" https://parts.igem.org/Part:BBa_K4806004">BBa_K4806004</a>), the HA-tag (<a href=" https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>)<sup>*</sup> for detection and the tRPL23-terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>)<sup>*</sup>. This part is codon-optimized for <i>Chlamydomonas reinhardtii</i> and was built as part of the CYPurify Collection. This level 2 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015).</p>
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<h2>Construct</h2>
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  <img class="bild" src="https://static.igem.wiki/teams/4806/wiki/registry/level2/cyp9q3-ha-construct.png">
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  <div class="unterschrift"><b>Fig.1 Construct design</b><br>
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  This construct was designed using the modular cloning system (MoClo).</div>
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</p>
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  <p>The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022). </p>
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<p><br></p>
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K4806222 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
 
 
<partinfo>BBa_K4806222 parameters</partinfo>
 
<partinfo>BBa_K4806222 parameters</partinfo>
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<h2>Results</h2>

Revision as of 09:06, 21 September 2023


CYP9Q3 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick)


This composite part contains the AβSAP(i)-promotor (BBa_K4806013), the coding sequence of CYP9Q3 (BBa_K4806004), the HA-tag (BBa_K3002017)* for detection and the tRPL23-terminator (BBa_K3002006)*. This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. This level 2 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015).


Construct

Fig.1 Construct design
This construct was designed using the modular cloning system (MoClo).

The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1991
    Illegal PstI site found at 2171
    Illegal PstI site found at 2677
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1991
    Illegal PstI site found at 2171
    Illegal PstI site found at 2677
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2533
    Illegal XhoI site found at 530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1991
    Illegal PstI site found at 2171
    Illegal PstI site found at 2677
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1991
    Illegal PstI site found at 2171
    Illegal PstI site found at 2677
    Illegal NgoMIV site found at 2948
    Illegal NgoMIV site found at 3636
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Results