Difference between revisions of "Part:BBa K4586004"

 
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<partinfo>BBa_K4586004 short</partinfo>
 
<partinfo>BBa_K4586004 short</partinfo>
  
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The Cas protein, which performs the enzymatic activity required for DNA editing (helicase and nuclease), is the first of two main components that make up the CRISPR system, a member of adaptive immunity found in the majority of bacteria. This system has been modified in various ways to be one of the most effective systems for gene editing.
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==Part Description==
  
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This part codes for the type V-K CRISPR-Cas system effector protein Cas12k, that is characterized by naturally inactivated RuvC domain and associated with Tn7-like transposon for RNA-guided DNA or genome editing, Its protospacer adjacent motif (PAM) sequence is GGTT. that is essential for activating the enzymatic activity of the Cas protein, and the guide RNA is designed based on the PAM sequence site corresponding to the target gene.
  
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==Usage==
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This part is implemented in our design to be the main element of our therapeutic agent (Cargo) to cause apoptosis in the autoreactive B-cells that secrete ACPA. Its guide RNA is designed to drive the protein portion of the CRISPR Cas system Cas12k to the BAFF-R gene, which is essential for B-cell survival and maintenance.
  
 
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Revision as of 09:35, 20 September 2023


Cas12k

Part Description

This part codes for the type V-K CRISPR-Cas system effector protein Cas12k, that is characterized by naturally inactivated RuvC domain and associated with Tn7-like transposon for RNA-guided DNA or genome editing, Its protospacer adjacent motif (PAM) sequence is GGTT. that is essential for activating the enzymatic activity of the Cas protein, and the guide RNA is designed based on the PAM sequence site corresponding to the target gene.

Usage

This part is implemented in our design to be the main element of our therapeutic agent (Cargo) to cause apoptosis in the autoreactive B-cells that secrete ACPA. Its guide RNA is designed to drive the protein portion of the CRISPR Cas system Cas12k to the BAFF-R gene, which is essential for B-cell survival and maintenance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]