Difference between revisions of "Part:BBa K4891015:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Using this protein, glucose could be transported into intracellular from extracellular.
+
Glucose facilitator serves as an alternative for enhancing glucose transportation rate. Then, glucose could be transported into intracellular from extracellular.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Glucokinase encoded by Glk and Glf from Zymomonas mobilis is synthesized.
+
Glucokinase encoded by glk and glf gene from Zymomonas mobilis is codon-optimized and synthesized.
  
 
===References===
 
===References===

Latest revision as of 01:47, 20 September 2023


ptsGup-glk-glf-ptsGdown


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 630
    Illegal PstI site found at 1642
    Illegal PstI site found at 1833
    Illegal PstI site found at 2406
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 630
    Illegal PstI site found at 1642
    Illegal PstI site found at 1833
    Illegal PstI site found at 2406
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 630
    Illegal PstI site found at 1642
    Illegal PstI site found at 1833
    Illegal PstI site found at 2406
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 630
    Illegal PstI site found at 1642
    Illegal PstI site found at 1833
    Illegal PstI site found at 2406
    Illegal AgeI site found at 927
    Illegal AgeI site found at 1818
    Illegal AgeI site found at 1974
    Illegal AgeI site found at 1986
    Illegal AgeI site found at 2697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glucose facilitator serves as an alternative for enhancing glucose transportation rate. Then, glucose could be transported into intracellular from extracellular.


Source

Glucokinase encoded by glk and glf gene from Zymomonas mobilis is codon-optimized and synthesized.

References