Difference between revisions of "Part:BBa K4891013:Design"

 
 
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===Design Notes===
 
===Design Notes===
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These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9.
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These recombinant plasmids pBAD33-ldhA/adhE/poxB/pta and pTargetF-sgRNAldhA/adhE/poxB/pta serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
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+
  
  
 
===Source===
 
===Source===
  
−
Recombinant plasmids pTargetF-sgRNAaroK/aroL/ptsH/ptsI initiate with pTargetF, which stores in our lab.
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ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655.
  
 
===References===
 
===References===

Latest revision as of 01:26, 20 September 2023


pta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These recombinant plasmids pBAD33-ldhA/adhE/poxB/pta and pTargetF-sgRNAldhA/adhE/poxB/pta serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.


Source

ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655.

References