Difference between revisions of "Part:BBa K243012:Design"

Revision as of 16:14, 16 October 2009

Strep-DigA-Middle Linker-Fok_i

Design Notes

The choice of the linker, lipocalin tag and purification tag allows us serval different combinations.

Source

Combined the parts by serial cloning steps. LOCUS Strep+Dig+middel 3625 bp DNA circular SYN 13-OCT-2009 DEFINITION Ligation of Strep+Dig+middellinker+FokI into pMA2_NgoMIV_SpeI ACCESSION Strep+Dig+middel KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3625) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI Fragment ends: SpeI and NgoMIV Fragment size: 2412 Insert molecule: Strep+Dig+middellinker+FokI Fragment ends: NgoMIV and SpeI Fragment size: 1213 FEATURES Location/Qualifiers CDS 129..989 /gene="AmpR" /SECDrawAs="Gene" CDS 2008..3222 /gene="ORF-1" /SECDrawAs="Gene" CDS 2014..2041 /gene="'Strep-Tag'" /SECDrawAs="Gene" CDS 2044..2599 /gene="'Dig'" /SECDrawAs="Gene" CDS 2602..2628 /gene="'middelLinker38" /SECDrawAs="Gene" CDS 2632..3219 /gene="'FokI" /SECDrawAs="Gene" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag 1981 aggtaccgaa ttcgcggccg cttctagatg gccggctgga gccatccgca gtttgaaaaa 2041 accggcgacg tgtaccacga cggcgcctgc cccgaagtga agcccgtgga caacttcgac 2101 tggtcccagt accacggcaa gtggtggcag gtggccgctt atcccgacca catcaccaag 2161 tacggcaagt gcggctgggc cgagtacacc cccgagggca agagcgtgaa ggtgtcccgg 2221 tacagcgtga tccacggcaa agagtacttc agcgagggca ccgcctaccc tgtgggcgac 2281 agcaagatcg gcaagatcta ccacagctac accatcggcg gcgtgaccca ggtgggcgtg 2341 agcaacgtgc tgtccaccga caacaagaac tacatcatcg gctacttttg cagatacgac 2401 gaggacaaga agggccactg ggacgccgtg tgggtgctgt cccggtccat ggtgctgacc 2461 ggcgaggcca agaccgccgt ggagaactac ctgatcggca gccccgtggt ggacagccag 2521 aaactggtgt acagcgactt ctccgaggcc gcctgcaaag tgaacaacag caactggtcc 2581 cacccccagt tcgaaaagac cggcggtggt tctggtggtg gttctggtac cggcaaatct 2641 gaactggagg agaaaaaatc cgagctgcgc cacaaactga aatatgtgcc tcacgagtat 2701 atcgaactga tcgagatcgc ccgtaatagt acccaagacc gtatcctgga aatgaaagtg 2761 atggagttct tcatgaaagt ctatggctat cgtggcaaac atctgggtgg tagccgtaaa 2821 ccagcaggtg ccatttatac cgttggttcc ccgatcgatt atggcgttat cgtggccaca 2881 aaagcgtatt ctggcggtta taatctgccg attggtcagg ctgatgagat ggaacgttat 2941 gtggaagaga atcagacccg taacaaacat ctgaacccga acgaatggtg gaaagtgtat 3001 ccgtcaagtg tcaccgagtt caaatttctg ttcgtgagcg gccactttaa aggcaactat 3061 aaagcccagc tgactcgtct gaaccatatc accaatagca atggggcagt gctgagtgtt 3121 gaggaactgc tgatcggtgg agaaatgatc aaagcaggca ccctgactct ggaagaagtt 3181 cgccgtaaat tcaacaatgg cgagatcaat tttaccggtt aatactagta gcggccgctg 3241 cagagctcat ggcgcgccta ggccttgacg gccttccgcc aattcgccct atagtgagtc 3301 gtattacgtc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 3361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 3421 aggcccgcac cgaaacgccc ttcccaacag ttgcgcagcc tgaatggcga atgggagcgc 3481 cctgtagcgg ccactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 3541 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 3601 caaaatatta acgcttacaa tttag // ===References===