Difference between revisions of "Part:BBa K4891008:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9.
+
The recombinant plasmids pBAD33-ldhA serves as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
  
  
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===Source===
 
===Source===
  
−
AroK and AroL (encoding shikimic acid kinase), PtsH and PtsI (encoding phosphotransferase system) are obtained from the genome of E. coli MG1655.
+
ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655.
  
 
===References===
 
===References===

Latest revision as of 09:23, 19 September 2023


ldhA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The recombinant plasmids pBAD33-ldhA serves as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.


Source

ldhA (encoding lactate dehydrogenase), adhE (encoding alcohol dehydrogenase), poxB (encoding pyruvate dehydrogenase) and pta (encoding phosphate acetyltransferase) are obtained from the genome of E. coli MG1655.

References