Difference between revisions of "Part:BBa K4891011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9. | + | These recombinant plasmids pTargetF-sgRNAaroK/aroL serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively. |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab. | |
===References=== | ===References=== | ||
Latest revision as of 09:19, 19 September 2023
sgRNAaroL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These recombinant plasmids pTargetF-sgRNAaroK/aroL serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
Source
AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.