Difference between revisions of "Part:BBa K4891007:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9.
+
These recombinant plasmids pBAD33-aroL serves as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
  
  
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===Source===
 
===Source===
  
−
AroK and AroL (encoding shikimic acid kinase), PtsH and PtsI (encoding phosphotransferase system) are obtained from the genome of E. coli MG1655.
+
AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.
  
 
===References===
 
===References===

Latest revision as of 09:15, 19 September 2023


aroL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 425
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 215
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 425
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 425
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These recombinant plasmids pBAD33-aroL serves as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.


Source

AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.

References