Difference between revisions of "Part:BBa K4891006:Design"
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | These recombinant plasmids pBAD33-aroK | + | These recombinant plasmids pBAD33-aroK serves as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively. |
Latest revision as of 09:11, 19 September 2023
aroK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 200
Design Notes
These recombinant plasmids pBAD33-aroK serves as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
Source
AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.