Difference between revisions of "Part:BBa K4606010"
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==Description==
 
==Description==
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    <p>For detailed design information, you can look up the registry of BBa_K4606007, BBa_K4606001 and BBa_K4606005.</p>
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    <p>Here, we loaded the genes encoding miR-3074-5p into plasmid vectors. In this way we were able to over-express the miRNA in the target cells.</p>
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    <p>Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin.</p>
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    <p>ZsGreen protein is spontaneously expressed and emits green fluorescence after successful transfection, so we can observe the transfection efficiency of the vector.</p>
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    <div style="margin:auto; width: 400px">   
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        <img src="https://static.igem.wiki/teams/4606/wiki/part-mir-3074-5p-map.jpg" width="400px">
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    </div>
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    <div style="margin:auto; width:400px;">
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        <p>Figure 1. Expression map of the composite plasmids of miR-3074-5p.</p>
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    </div>
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Revision as of 02:27, 19 September 2023


Vector-ZsGreen-miR-3074-5p-AmpR

Description

For detailed design information, you can look up the registry of BBa_K4606007, BBa_K4606001 and BBa_K4606005.

Here, we loaded the genes encoding miR-3074-5p into plasmid vectors. In this way we were able to over-express the miRNA in the target cells.

Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin.

ZsGreen protein is spontaneously expressed and emits green fluorescence after successful transfection, so we can observe the transfection efficiency of the vector.

Figure 1. Expression map of the composite plasmids of miR-3074-5p.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]