Difference between revisions of "Part:BBa K4606004"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4606004 short</partinfo> | ||
+ | |||
+ | ==Description== | ||
+ | <html> | ||
+ | <p>For detailed design information, you can look up the registry of <a href="https://parts.igem.org/Part:BBa_K4606003">BBa_K4606003</a>, the device for gene segment of SMAD4.</p> | ||
+ | <p>According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.</p> | ||
+ | <div style="margin:auto; width: 400px"> | ||
+ | <img src="https://static.igem.wiki/teams/4606/wiki/part-4-smad4-mut.jpg" width="400px"> | ||
+ | </div> | ||
+ | <div style="margin:auto; width:400px;"> | ||
+ | <p>Figure3 (Part4). Acquisition of SMAD4-MUT gene segments by PCR. | ||
+ | <br>1#: DNA marker. 2#: Segments of target gene. | ||
+ | </p> | ||
+ | </div> | ||
+ | </html> | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4606004 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4606004 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 13:12, 18 September 2023
SMAD4(mutant type)
Description
For detailed design information, you can look up the registry of BBa_K4606003, the device for gene segment of SMAD4.
According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.
Figure3 (Part4). Acquisition of SMAD4-MUT gene segments by PCR.
1#: DNA marker. 2#: Segments of target gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]