Difference between revisions of "Part:BBa K4806001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts. <br> | + | This part has been codon optimized for <i>Chlamydomonas reinhardtii</i> and it is compatible with Phytobricks/MoClo parts. <br> |
We had to insert one mutation at 586 bp to fit to the MoClo standards of iGEM. In our lab we are not using the restriction enzyme <i>Sap</i>I, but instead <i>Bbs</i>I. Therefore, this mutation does not occure in the parts we used in our experiments. This mutation is silent, but it might affect the expression level, since the triplet AGT is much rarer in <i>Chlamydomonas</i>, compared to the triplet AGC, which we used. | We had to insert one mutation at 586 bp to fit to the MoClo standards of iGEM. In our lab we are not using the restriction enzyme <i>Sap</i>I, but instead <i>Bbs</i>I. Therefore, this mutation does not occure in the parts we used in our experiments. This mutation is silent, but it might affect the expression level, since the triplet AGT is much rarer in <i>Chlamydomonas</i>, compared to the triplet AGC, which we used. | ||
Revision as of 07:47, 18 September 2023
CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 356
Illegal PstI site found at 1712
Illegal PstI site found at 2145 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 356
Illegal PstI site found at 1712
Illegal PstI site found at 2145
Illegal NotI site found at 862 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 356
Illegal PstI site found at 1712
Illegal PstI site found at 2145 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 356
Illegal PstI site found at 1712
Illegal PstI site found at 2145
Illegal NgoMIV site found at 999
Illegal NgoMIV site found at 1348 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.
We had to insert one mutation at 586 bp to fit to the MoClo standards of iGEM. In our lab we are not using the restriction enzyme SapI, but instead BbsI. Therefore, this mutation does not occure in the parts we used in our experiments. This mutation is silent, but it might affect the expression level, since the triplet AGT is much rarer in Chlamydomonas, compared to the triplet AGC, which we used.
Source
https://www.uniprot.org/uniprotkb/P10635/entry