Difference between revisions of "Part:BBa K4891002:Design"

 
 
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===Design Notes===
 
===Design Notes===
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For one thing, the usage of AroGfbr could alleviate the feedback inhibition of aromatic amino acids. For another thing, AroB optimization enable to enhance expression level. As a result, shikimate biosynthesis is improved owing to the enhanced metabolic flow.
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Improving expression level of aroD gene by recombinant plasmid is beneficial for accelerating metabolic rate, and thus enhances shikimate biosynthesis.
  
  
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===Source===
 
===Source===
  
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There are four genes for converting PEP and E4P into shikimate, including aroG (encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase), aroB (encoding 3-dehydroquinate synthase), aroD (encoding 3-dehydroquinate dehydratase), and aroE (encoding shikimate dehydrogenase), and these genes originate from Escherichia coli.
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This gene is originated from Escherichia coli MG1655.
  
 
===References===
 
===References===

Latest revision as of 06:35, 18 September 2023


aroD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 21
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 208


Design Notes

Improving expression level of aroD gene by recombinant plasmid is beneficial for accelerating metabolic rate, and thus enhances shikimate biosynthesis.


Source

This gene is originated from Escherichia coli MG1655.

References