Difference between revisions of "Part:BBa K4605005"
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To explore whether more colored fibers could be expressed in K.xylinus. We wanted to express EGFP in K.xylinus using a strong promoter (J23100) as a way to get fluorescent cellulose membranes. | To explore whether more colored fibers could be expressed in K.xylinus. We wanted to express EGFP in K.xylinus using a strong promoter (J23100) as a way to get fluorescent cellulose membranes. | ||
==Experiments== | ==Experiments== | ||
| + | We chose to use the Anderson family promoter, first, because it is compatible with K.xylinus, and there is enough references to report that these promoters have been tested in several of bacteria such as G. xylinus 700178, G. hansenii | ||
| + | 53582, and K. rhaeticus iGEM.Then we selected three of the strong promoters:J23100,J23104 and J23119.We linked it to bpsA in the hope that fluorescent cellulose could be expressed. | ||
<html><style> | <html><style> | ||
img{} | img{} | ||
Revision as of 03:25, 18 September 2023
Used to produce fluorescent bacterial cellulose
Description
To explore whether more colored fibers could be expressed in K.xylinus. We wanted to express EGFP in K.xylinus using a strong promoter (J23100) as a way to get fluorescent cellulose membranes.
Experiments
We chose to use the Anderson family promoter, first, because it is compatible with K.xylinus, and there is enough references to report that these promoters have been tested in several of bacteria such as G. xylinus 700178, G. hansenii 53582, and K. rhaeticus iGEM.Then we selected three of the strong promoters:J23100,J23104 and J23119.We linked it to bpsA in the hope that fluorescent cellulose could be expressed.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]