Difference between revisions of "Part:BBa K4719009"

 
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<partinfo>BBa_K4719009 short</partinfo>
 
<partinfo>BBa_K4719009 short</partinfo>
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===Introduction===
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Vilnius Lithuania iGEM 2023 team's goal was to create a universal synthetic biology system in ''Komagataeibacter xylinus'' for ''in vivo'' bacterial cellulose polymer composition modification. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry.
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Colorful cellulose was made by introducing styrene monooxygenase pKARA_RT3 [https://parts.igem.org/Part:BBa_K4719018 BBa_K4719018] to ''K. xylinus''. This enzyme can metabolize indigo and its other derivatives into indigo dyes. Bacteria produce cellulose alongside pigments. Since they are not water soluble, the final product retains the color.
  
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flavin dependent monooxygenase
 
  
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===Usage and Biology===
 
===Usage and Biology===
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pKARA_RT3 is styrene monooxygenase. Styrene monooxygenases belong to oxidoreductases, specifically to an enzyme class 1.14.14-, which act on paired donors, with incorporation or reduction of molecular oxygen, which is donated by reduced flavin or flavoprotein. Styrene monooxygenases have been shown to produce indigo - a blue dye [https://parts.igem.org/Part:BBa_K4719009#references (1)].
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===References===
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1. O’Connor, K.E., Dobson, A.D. and Hartmans, S. (1997) ‘Indigo formation by microorganisms expressing styrene monooxygenase activity’, Applied and Environmental Microbiology, 63(11), pp. 4287–4291. doi:10.1128/aem.63.11.4287-4291.1997.
  
 
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Revision as of 14:18, 17 September 2023


pKARA_RT3 styrene monooxigenase

Introduction

Vilnius Lithuania iGEM 2023 team's goal was to create a universal synthetic biology system in Komagataeibacter xylinus for in vivo bacterial cellulose polymer composition modification. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry.

Colorful cellulose was made by introducing styrene monooxygenase pKARA_RT3 BBa_K4719018 to K. xylinus. This enzyme can metabolize indigo and its other derivatives into indigo dyes. Bacteria produce cellulose alongside pigments. Since they are not water soluble, the final product retains the color.


Usage and Biology

pKARA_RT3 is styrene monooxygenase. Styrene monooxygenases belong to oxidoreductases, specifically to an enzyme class 1.14.14-, which act on paired donors, with incorporation or reduction of molecular oxygen, which is donated by reduced flavin or flavoprotein. Styrene monooxygenases have been shown to produce indigo - a blue dye (1).

References

1. O’Connor, K.E., Dobson, A.D. and Hartmans, S. (1997) ‘Indigo formation by microorganisms expressing styrene monooxygenase activity’, Applied and Environmental Microbiology, 63(11), pp. 4287–4291. doi:10.1128/aem.63.11.4287-4291.1997.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1171
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1171
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1171
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1171
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 448
    Illegal AgeI site found at 634
  • 1000
    COMPATIBLE WITH RFC[1000]