Difference between revisions of "Part:BBa K4719003"

Revision as of 16:53, 16 September 2023

UAP1

Introduction

Vilnius Lithuania iGEM 2023 team's goal was to create a universal synthetic biology system in Komagataeibacter xylinus for in vivo bacterial cellulose polymer composition modification. Firstly, we chose to produce a cellulose-chitin polymer that would later be deacetylated, creating bacterial cellulose-chitosan. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design.

Bacterial cellulose-chitin polymer was achieved by increasing the production of UDP-N-acetylglucosamine (UDP-GlcNAc), which can be recognized as a viable substrate for cellulose synthase and incorporated in the bacterial cellulose polymer. We employed two strategies to produce this material. The first approach was to add N-acetylglucosamine into the growth medium BBa_K4719013, and the second one was the production of N-acetylglucosamine by K. xylinus from simple sugars such as glucose, fructose, and saccharose in the growth medium BBa_K4719014.

Usage and Biology

UAP1 is UDP-GlcNAc pyrophosphorylase. This protein is involved in UDP-GlcNAc synthesis by converting N-acetylglucosamine-6-phosphate into UDP-GlcNAc. The cellulose synthase can recognize both UDP-N-acetylglucosamine and UDP-glucose as substrates resulting in the formation of bacterial cellulose-chitin polymer. This part is used in BBa_K4719013 and BBa_K4719014.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 573
Illegal EcoRI site found at 735
Illegal SpeI site found at 511
Illegal SpeI site found at 1399
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 573
Illegal EcoRI site found at 735
Illegal SpeI site found at 511
Illegal SpeI site found at 1399
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 573
Illegal EcoRI site found at 735
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 573
Illegal EcoRI site found at 735
Illegal SpeI site found at 511
Illegal SpeI site found at 1399
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 573
Illegal EcoRI site found at 735
Illegal SpeI site found at 511
Illegal SpeI site found at 1399
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 <br>
 <br>
-Bacterial cellulose-chitin polymer was achieved by increasing the production of UDP-N-acetylglucosamine (UDP-GlcNAc), which can be recognized as a viable substrate for cellulose synthase and incorporated in bacterial cellulose polymer. We employed two strategies to produce this material. The first approach was to add N-acetylglucosamine into the growth medium [https://parts.igem.org/Part:BBa_K4719013 BBa_K4719013], and the second one was the production of N-acetylglucosamine by ''K. xylinus'' from simple sugars such as glucose, fructose, and saccharose in the growth medium [https://parts.igem.org/Part:BBa_K4719014 BBa_K4719014].
+Bacterial cellulose-chitin polymer was achieved by increasing the production of UDP-N-acetylglucosamine (UDP-GlcNAc), which can be recognized as a viable substrate for cellulose synthase and incorporated in the bacterial cellulose polymer. We employed two strategies to produce this material. The first approach was to add N-acetylglucosamine into the growth medium [https://parts.igem.org/Part:BBa_K4719013 BBa_K4719013], and the second one was the production of N-acetylglucosamine by ''K. xylinus'' from simple sugars such as glucose, fructose, and saccharose in the growth medium [https://parts.igem.org/Part:BBa_K4719014 BBa_K4719014].
@@ Line 13: / Line 13: @@
 ===Usage and Biology===
-UAP1 is UDP-GlcNAc pyrophosphorylase. This protein is involved in UDP-GlcNAc synthesis by converting N-acetylglucosamine-6-phosphate into UDP-GlcNAc. The cellulose synthase can recognize both UDP-N-acetylglucosamine and UDP-glucose as substrates resulting in the formation of bacterial cellulose-chitin polymer. This part is used in [https://parts.igem.org/Part:BBa_K4719013 BBa_K4719013] and [https://parts.igem.org/Part:BBa_K4719014 BBa_K4719014].
+''UAP1'' is UDP-GlcNAc pyrophosphorylase. This protein is involved in UDP-GlcNAc synthesis by converting N-acetylglucosamine-6-phosphate into UDP-GlcNAc. The cellulose synthase can recognize both UDP-N-acetylglucosamine and UDP-glucose as substrates resulting in the formation of bacterial cellulose-chitin polymer. This part is used in [https://parts.igem.org/Part:BBa_K4719013 BBa_K4719013] and [https://parts.igem.org/Part:BBa_K4719014 BBa_K4719014].