Difference between revisions of "Part:BBa K4606007"
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In this part, we designed a plasmid for fusion protein recombination. With this device, we can insert the coding sequence of a novel protein before Poly A. When transfected into E. coli or other recipient cells, it amplifies and expresses the corresponding gene product. Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin. To use this device, all you need to do is cut the plasmid at Xba1 and insert your target fragment into this position. We have proved its feasibility by inserting our destination fragment (Firefly Luciferase) to the plasmid. The recombinants show resistance to Ampicillin. | In this part, we designed a plasmid for fusion protein recombination. With this device, we can insert the coding sequence of a novel protein before Poly A. When transfected into E. coli or other recipient cells, it amplifies and expresses the corresponding gene product. Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin. To use this device, all you need to do is cut the plasmid at Xba1 and insert your target fragment into this position. We have proved its feasibility by inserting our destination fragment (Firefly Luciferase) to the plasmid. The recombinants show resistance to Ampicillin. | ||
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Revision as of 09:05, 16 September 2023
Vector-AmpR
In this part, we designed a plasmid for fusion protein recombination. With this device, we can insert the coding sequence of a novel protein before Poly A. When transfected into E. coli or other recipient cells, it amplifies and expresses the corresponding gene product. Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin. To use this device, all you need to do is cut the plasmid at Xba1 and insert your target fragment into this position. We have proved its feasibility by inserting our destination fragment (Firefly Luciferase) to the plasmid. The recombinants show resistance to Ampicillin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]