Difference between revisions of "Part:BBa K243003:Design"

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KEYWORDS    .
 
KEYWORDS    .
 
SOURCE      Unknown.
 
SOURCE      Unknown.
−
  ORGANISM Unknown
+
ORGANISM   Unknown
 
             Unclassified.
 
             Unclassified.
 
REFERENCE  1  (bases 1 to 3616)
 
REFERENCE  1  (bases 1 to 3616)

Revision as of 15:01, 16 October 2009

Digoxigenin binding protein (DigA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Freiburg standard25 part

Source

synthesized by purimex

LOCUS His+FluA+Split+F 3616 bp DNA circular SYN 08-OCT-2009 DEFINITION Ligation of His+FluA+Split+FokI into pMA2_NgoMIV_SpeI ACCESSION His+FluA+Split+F KEYWORDS . SOURCE Unknown. ORGANISM Unknown 

           Unclassified.

REFERENCE 1 (bases 1 to 3616) 

 AUTHORS   Self
 JOURNAL   Unpublished.

COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI 

              Fragment ends: SpeI and NgoMIV
              Fragment size: 2412
           Insert molecule:  His+FluA+Split+FokI
              Fragment ends: NgoMIV and SpeI
              Fragment size: 1204

FEATURES Location/Qualifiers 

    CDS             129..989
                    /gene="AmpR"
                    /SECDrawAs="Gene"
    CDS             2008..3213
                    /gene="ORF-1"
                    /SECDrawAs="Gene"
    CDS             2017..2034
                    /gene="His Tag"
                    /SECDrawAs="Gene"
    CDS             2041..2562
                    /gene="FluA"
                    /SECDrawAs="Gene"
    CDS             2569..2619
                    /gene="Split-Linker"
                    /SECDrawAs="Gene"
    CDS             2623..3212
                    /gene="'Fok inaktiv'"
                    /SECDrawAs="Gene"

ORIGIN 

       1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga
    1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga
    1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag
    1981 aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc
    2041 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc
    2101 cagtaccacg gcaagtggtg ggaggtggcc aagtacccca gccccaacgg caagtatggc
    2161 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc cagatacgac
    2221 gtgatccacg gcaaagaata cttcatggaa ggcaccgcct accccgtggg cgacagcaag
    2281 atcggcaaga tctaccacag ccggaccgtg ggcggctaca ccagaaagac cgtgttcaac
    2341 gtgctgtcca ccgacaacaa gaactacatc atcggctaca gctgccgcta cgacgaggac
    2401 aagaagggcc actgggacca cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag
    2461 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg
    2521 gtgtacagcg acttcagcga ggccgcctgc aaagtgaaca acaccggccg accagcctgt
    2581 aagattccaa atgacctgaa gcagaaagtt atgaatcaca ccggcaaatc tgaactggag
    2641 gagaaaaaat ccgagctgcg ccacaaactg aaatatgtgc ctcacgagta tatcgaactg
    2701 atcgagatcg cccgtaatag tacccaagac cgtatcctgg aaatgaaagt gatggagttc
    2761 ttcatgaaag tctatggcta tcgtggcaaa catctgggtg gtagccgtaa accagcaggt
    2821 gccatttata ccgttggttc cccgatcgat tatggcgtta tcgtggccac aaaagcgtat
    2881 tctggcggtt ataatctgcc gattggtcag gctgatgaga tggaacgtta tgtggaagag
    2941 aatcagaccc gtaacaaaca tctgaacccg aacgaatggt ggaaagtgta tccgtcaagt
    3001 gtcaccgagt tcaaatttct gttcgtgagc ggccacttta aaggcaacta taaagcccag
    3061 ctgactcgtc tgaaccatat caccaatagc aatggggcag tgctgagtgt tgaggaactg
    3121 ctgatcggtg gagaaatgat caaagcaggc accctgactc tggaagaagt tcgccgtaaa
    3181 ttcaacaatg gcgagatcaa ttttaccggt taatactagt agcggccgct gcagagctca
    3241 tggcgcgcct aggccttgac ggccttccgc caattcgccc tatagtgagt cgtattacgt
    3301 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
    3361 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    3421 ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg
    3481 gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    3541 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    3601 aacgcttaca atttag

//

References