Difference between revisions of "Part:BBa K203112:Design"
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Sequence was adapted from [1] to include a HindIII site between core and proximal promoter (after base pair 66). Sequence was then synthesized by Assembly PCR [2] using Oligos designed using [3]: | Sequence was adapted from [1] to include a HindIII site between core and proximal promoter (after base pair 66). Sequence was then synthesized by Assembly PCR [2] using Oligos designed using [3]: | ||
| − | JeT 01 GTACCAATTGGTGAGAATTCACTAGTGGGCGGAGTTA | + | JeT 01 GTACCAATTGGTGAGAATTCACTAGTGGGCGGAGTTA<br> |
| − | JeT 02 CGCTGATTGGCTCCGCCCTAACTCCGCCCACTAGTGA | + | JeT 02 CGCTGATTGGCTCCGCCCTAACTCCGCCCACTAGTGA<br> |
| − | JeT 03 AGGGCGGAGCCAATCAGCGTGCGCCGTTCCGAAAGTT | + | JeT 03 AGGGCGGAGCCAATCAGCGTGCGCCGTTCCGAAAGTT<br> |
| − | JeT 04 CGCCCAGCCATAAAAGGCAACTTTCGGAACGGCGCAC | + | JeT 04 CGCCCAGCCATAAAAGGCAACTTTCGGAACGGCGCAC<br> |
| − | JeT 05 TGCCTTTTATGGCTGGGCGGAAGCTTAGAATGGGCGG | + | JeT 05 TGCCTTTTATGGCTGGGCGGAAGCTTAGAATGGGCGG<br> |
| − | JeT 06 TATAATCATCGGCGTTCACCGCCCATTCTAAGCTTCC | + | JeT 06 TATAATCATCGGCGTTCACCGCCCATTCTAAGCTTCC<br> |
| − | JeT 07 GTGAACGCCGATGATTATATAAGGACGCGCCGGGTGT | + | JeT 07 GTGAACGCCGATGATTATATAAGGACGCGCCGGGTGT<br> |
| − | JeT 08 GACGGAACTAGCTGTGCCACACCCGGCGCGTCCTTAT | + | JeT 08 GACGGAACTAGCTGTGCCACACCCGGCGCGTCCTTAT<br> |
| − | JeT 09 TGGCACAGCTAGTTCCGTCGCAGCCGGGATTTGGGTCG | + | JeT 09 TGGCACAGCTAGTTCCGTCGCAGCCGGGATTTGGGTCG<br> |
| − | JeT 10 CTAGCACAAACAAGAACCGCGACCCAAATCCCGGCTGC | + | JeT 10 CTAGCACAAACAAGAACCGCGACCCAAATCCCGGCTGC<br> |
| − | JeT 11 CGGTTCTTGTTTGTGCTAGCCTGCAGGTCAGAGATTGA | + | JeT 11 CGGTTCTTGTTTGTGCTAGCCTGCAGGTCAGAGATTGA<br> |
JeT 12 AGTCTGATCAATCTCTGACCTGCAGG | JeT 12 AGTCTGATCAATCTCTGACCTGCAGG | ||
Revision as of 14:49, 16 October 2009
JeT; constitutive promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was adapted from [1] to include a HindIII site between core and proximal promoter (after base pair 66). Sequence was then synthesized by Assembly PCR [2] using Oligos designed using [3]:
JeT 01 GTACCAATTGGTGAGAATTCACTAGTGGGCGGAGTTA
JeT 02 CGCTGATTGGCTCCGCCCTAACTCCGCCCACTAGTGA
JeT 03 AGGGCGGAGCCAATCAGCGTGCGCCGTTCCGAAAGTT
JeT 04 CGCCCAGCCATAAAAGGCAACTTTCGGAACGGCGCAC
JeT 05 TGCCTTTTATGGCTGGGCGGAAGCTTAGAATGGGCGG
JeT 06 TATAATCATCGGCGTTCACCGCCCATTCTAAGCTTCC
JeT 07 GTGAACGCCGATGATTATATAAGGACGCGCCGGGTGT
JeT 08 GACGGAACTAGCTGTGCCACACCCGGCGCGTCCTTAT
JeT 09 TGGCACAGCTAGTTCCGTCGCAGCCGGGATTTGGGTCG
JeT 10 CTAGCACAAACAAGAACCGCGACCCAAATCCCGGCTGC
JeT 11 CGGTTCTTGTTTGTGCTAGCCTGCAGGTCAGAGATTGA
JeT 12 AGTCTGATCAATCTCTGACCTGCAGG
Source
To be up dated