Difference between revisions of "Part:BBa K4606003:Design"
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<partinfo>BBa_K4606003 short</partinfo> | <partinfo>BBa_K4606003 short</partinfo> | ||
| + | SMAD4 is important for cartilage homeostasis. The inhibition of SMAD4 can induce chondrocyte apoptosis and aggravate osteoarthritis progression. Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in the course of osteoarthritis. | ||
| + | We constructed a portion of the gene encoding SMAD4. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment. This fusion expression does nearly no harm to the normal physiological state of firely luciferase. | ||
<partinfo>BBa_K4606003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4606003 SequenceAndFeatures</partinfo> | ||
Revision as of 13:57, 14 September 2023
SMAD4(wild type)
SMAD4 is important for cartilage homeostasis. The inhibition of SMAD4 can induce chondrocyte apoptosis and aggravate osteoarthritis progression. Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in the course of osteoarthritis.
We constructed a portion of the gene encoding SMAD4. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment. This fusion expression does nearly no harm to the normal physiological state of firely luciferase.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
AAA
Source
AAA