Difference between revisions of "Part:BBa K4606000"
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<partinfo>BBa_K4606000 short</partinfo>
 
<partinfo>BBa_K4606000 short</partinfo>
  
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In this part, we designed a plasmid for fusion protein recombination. With this device, we can display the coding sequence of a novel protein before Poly A. When transfected into E. coli or other recipient cells, it amplifies and expresses the corresponding gene product.
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In this part, we designed a plasmid for fusion protein recombination. With this device, we can insert the coding sequence of a novel protein before Poly A. When transfected into E. coli or other recipient cells, it amplifies and expresses the corresponding gene product.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:55, 14 September 2023


Vector-AmpR

In this part, we designed a plasmid for fusion protein recombination. With this device, we can insert the coding sequence of a novel protein before Poly A. When transfected into E. coli or other recipient cells, it amplifies and expresses the corresponding gene product.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1163
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1163
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1163
    Illegal NgoMIV site found at 487
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 279
    Illegal BsaI.rc site found at 2637
    Illegal SapI site found at 1557