Difference between revisions of "Part:BBa K180008:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K180008 short</partinfo>
 
<partinfo>BBa_K180008 short</partinfo>
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===Design Notes===
 
===Design Notes===
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PstI site within the mCherry sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest.
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PstI site within the mCherry sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.
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'''Part cannot be submitted to registry as the rights are owned by Clontech. The mCherry sequence is however commercially available from Clontech in the pmCherry vector.'''
  
  

Revision as of 14:05, 16 October 2009

mCherry (rights owned by Clontech)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PstI site within the mCherry sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.

Part cannot be submitted to registry as the rights are owned by Clontech. The mCherry sequence is however commercially available from Clontech in the pmCherry vector.


Source

pmCherry vector, Clontech

References