Difference between revisions of "Part:BBa K4806001:Design"
Line 3: Line 3:
 
<partinfo>BBa_K4806001 short</partinfo>
 
<partinfo>BBa_K4806001 short</partinfo>
  
−
<partinfo>BBa_K4806001 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K4806001 SequenceAndFeatures ggcctggaggccctggtgcccctggccgtgatcgtggccatcttcctgctgctggtggacctgatgcaccgccgccagcg
 +
ctgggcTgcTcgctacagcccTggTcccctgccTctgcccgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGC
 +
TTGCAGATTTGACTTGCAACGCCCGCATTGTGTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAA
 +
CCCTGCGTCGCCGTTTCCATTTGCAGgcctgggcaacctgctgcacgtggacttccagaacaccccctactgcttcgacc
 +
agctgcgccgccgcttcggTgacgtgttcagcctgcagctggcTtggaccccTgtggtggtgctgaacggcctggcTgcT
 +
gtgcgcgaggccctggtgacccacggcgaggacaccgccgaccgcccTccTgtgcccatcacccagatcctgggTttcgg
 +
TccTcgcagccagggcgtgttcctggcccgctacggTccTgcTtggcgcgagcagcgccgcttcagcgtgagcaccctgc
 +
gcaacctgggcctgggcaagaagagcctggagcagtgggtgaccgaggaggccgcctgcctgtgcgccgccttcgccaac
 +
cacagcgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGTGTC
 +
GACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccgcccc
 +
ttccgcccTaacggTctgctggacaaggccgtgagcaacgtgatcgccagcctgacctgcggccgccgcttcgagtacga
 +
cgacccccgcttcctgcgcctgctggacctggcccaggagggcctgaaggaggagagcggcttcctgcgcgaggtgctga
 +
acgccgtgcccgtgctgctgcacatccccgccctggccggcaaggtgctgcgcttccagaaggccttcctgacccagctg
 +
gacgagctgctgaccgagcaccgcatgacctgggacccTgcTcagccTccTcgcgacctgaccgaggccttcctggcTga
 +
gatggagaagGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGT
 +
GTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccaa
 +
gggcaaccccgagagcagcttcaacgacgagaacctgcgcatcgtggtggccgacctgttcagcgccggcatggtgacca
 +
ccagcaccaccctggcctggggcctgctgctgatgatcctgcaccccgacgtgcagcgccgcgtgcagcaggagatcgac
 +
gacgtgatcggccaggtgcgccgccccgagatgggcgaccaggcccacatgccctacaccaccgccgtgatccacgaggt
 +
gcagcgcttcggcgacatcgtgcccctgggcgtgacccacatgaccagccgcgacatcgaggtgcagggcttccgcatcc
 +
ccaagggcaccaccctgatcaccaacctgagcagcgtgctgaaggacgagGTGAGCTTGCGGGGTTGCGAGCAACACTCC
 +
AGCAACGAACAGTGCCCAAGTCAGGAATCTGCAGTCAGCCTGGGCTTTCGGCGGCTTTTTCTTGGGCAAACAGCTTGCAC
 +
TCATGCCAGCGCGGCTTGTCCAGCCTCACTTGAGCTTTCCAGCTGCTACCAGCCGGGCTATACGACAGCGACAGAGCCAT
 +
AGCGTGGAATCACTTATTTGGGTTGCCGAAGTAGCGGTCGGAGCGTGAGTTCTTGGTCAAGCCGCCCCTTATCCGGTTCC
 +
TGTCCGTGTCTTTGTCCCTCGTTCACCCTTCGCGGCACCCTTCATCCCCTTGCTTGCAGgccgtgtgggagaagcccttc
 +
cgcttccacccTgagcacttcctggacgcTcagggTcacttcgtgaagccTgaggccttcctgcccttcagcgcTggTcg
 +
ccgcgcctgcctgggcgagcccctggcccgcatggagctgttcctgttcttcaccagcctgctgcagcacttcagcttca
 +
gcgtggcTgcTggTcagccTcgccccagccacagccgcgtggtgagcttcctggtgaccccTagcccctacgagctgtgc
 +
gccgtgccccgcgg
 +
</partinfo>
  
 
ggcctggaggccctggtgcccctggccgtgatcgtggccatcttcctgctgctggtggacctgatgcaccgccgccagcg
 
ggcctggaggccctggtgcccctggccgtgatcgtggccatcttcctgctgctggtggacctgatgcaccgccgccagcg

Revision as of 12:26, 13 September 2023


CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
    Illegal NotI site found at 862
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 1348
  • 1000
    COMPATIBLE WITH RFC[1000]

ggcctggaggccctggtgcccctggccgtgatcgtggccatcttcctgctgctggtggacctgatgcaccgccgccagcg ctgggcTgcTcgctacagcccTggTcccctgccTctgcccgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGC TTGCAGATTTGACTTGCAACGCCCGCATTGTGTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAA CCCTGCGTCGCCGTTTCCATTTGCAGgcctgggcaacctgctgcacgtggacttccagaacaccccctactgcttcgacc agctgcgccgccgcttcggTgacgtgttcagcctgcagctggcTtggaccccTgtggtggtgctgaacggcctggcTgcT gtgcgcgaggccctggtgacccacggcgaggacaccgccgaccgcccTccTgtgcccatcacccagatcctgggTttcgg TccTcgcagccagggcgtgttcctggcccgctacggTccTgcTtggcgcgagcagcgccgcttcagcgtgagcaccctgc gcaacctgggcctgggcaagaagagcctggagcagtgggtgaccgaggaggccgcctgcctgtgcgccgccttcgccaac cacagcgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGTGTC GACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccgcccc ttccgcccTaacggTctgctggacaaggccgtgagcaacgtgatcgccagcctgacctgcggccgccgcttcgagtacga cgacccccgcttcctgcgcctgctggacctggcccaggagggcctgaaggaggagagcggcttcctgcgcgaggtgctga acgccgtgcccgtgctgctgcacatccccgccctggccggcaaggtgctgcgcttccagaaggccttcctgacccagctg gacgagctgctgaccgagcaccgcatgacctgggacccTgcTcagccTccTcgcgacctgaccgaggccttcctggcTga gatggagaagGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGT GTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccaa gggcaaccccgagagcagcttcaacgacgagaacctgcgcatcgtggtggccgacctgttcagcgccggcatggtgacca ccagcaccaccctggcctggggcctgctgctgatgatcctgcaccccgacgtgcagcgccgcgtgcagcaggagatcgac gacgtgatcggccaggtgcgccgccccgagatgggcgaccaggcccacatgccctacaccaccgccgtgatccacgaggt gcagcgcttcggcgacatcgtgcccctgggcgtgacccacatgaccagccgcgacatcgaggtgcagggcttccgcatcc ccaagggcaccaccctgatcaccaacctgagcagcgtgctgaaggacgagGTGAGCTTGCGGGGTTGCGAGCAACACTCC AGCAACGAACAGTGCCCAAGTCAGGAATCTGCAGTCAGCCTGGGCTTTCGGCGGCTTTTTCTTGGGCAAACAGCTTGCAC TCATGCCAGCGCGGCTTGTCCAGCCTCACTTGAGCTTTCCAGCTGCTACCAGCCGGGCTATACGACAGCGACAGAGCCAT AGCGTGGAATCACTTATTTGGGTTGCCGAAGTAGCGGTCGGAGCGTGAGTTCTTGGTCAAGCCGCCCCTTATCCGGTTCC TGTCCGTGTCTTTGTCCCTCGTTCACCCTTCGCGGCACCCTTCATCCCCTTGCTTGCAGgccgtgtgggagaagcccttc cgcttccacccTgagcacttcctggacgcTcagggTcacttcgtgaagccTgaggccttcctgcccttcagcgcTggTcg ccgcgcctgcctgggcgagcccctggcccgcatggagctgttcctgttcttcaccagcctgctgcagcacttcagcttca gcgtggcTgcTggTcagccTcgccccagccacagccgcgtggtgagcttcctggtgaccccTagcccctacgagctgtgc gccgtgccccgcgg


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii (https://www.kazusa.or.jp/codon/)
We inserted a few mutations to eliminate the recognition sites for our type IIS restriction enzymes BbsI and BsaI.


Source

https://www.uniprot.org/uniprotkb/P10635/entry

References