Difference between revisions of "Part:BBa K4806001:Design"
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<partinfo>BBa_K4806001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4806001 SequenceAndFeatures</partinfo> | ||
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| + | ggcctggaggccctggtgcccctggccgtgatcgtggccatcttcctgctgctggtggacctgatgcaccgccgccagcg | ||
| + | ctgggcTgcTcgctacagcccTggTcccctgccTctgcccgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGC | ||
| + | TTGCAGATTTGACTTGCAACGCCCGCATTGTGTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAA | ||
| + | CCCTGCGTCGCCGTTTCCATTTGCAGgcctgggcaacctgctgcacgtggacttccagaacaccccctactgcttcgacc | ||
| + | agctgcgccgccgcttcggTgacgtgttcagcctgcagctggcTtggaccccTgtggtggtgctgaacggcctggcTgcT | ||
| + | gtgcgcgaggccctggtgacccacggcgaggacaccgccgaccgcccTccTgtgcccatcacccagatcctgggTttcgg | ||
| + | TccTcgcagccagggcgtgttcctggcccgctacggTccTgcTtggcgcgagcagcgccgcttcagcgtgagcaccctgc | ||
| + | gcaacctgggcctgggcaagaagagcctggagcagtgggtgaccgaggaggccgcctgcctgtgcgccgccttcgccaac | ||
| + | cacagcgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGTGTC | ||
| + | GACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccgcccc | ||
| + | ttccgcccTaacggTctgctggacaaggccgtgagcaacgtgatcgccagcctgacctgcggccgccgcttcgagtacga | ||
| + | cgacccccgcttcctgcgcctgctggacctggcccaggagggcctgaaggaggagagcggcttcctgcgcgaggtgctga | ||
| + | acgccgtgcccgtgctgctgcacatccccgccctggccggcaaggtgctgcgcttccagaaggccttcctgacccagctg | ||
| + | gacgagctgctgaccgagcaccgcatgacctgggacccTgcTcagccTccTcgcgacctgaccgaggccttcctggcTga | ||
| + | gatggagaagGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGT | ||
| + | GTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccaa | ||
| + | gggcaaccccgagagcagcttcaacgacgagaacctgcgcatcgtggtggccgacctgttcagcgccggcatggtgacca | ||
| + | ccagcaccaccctggcctggggcctgctgctgatgatcctgcaccccgacgtgcagcgccgcgtgcagcaggagatcgac | ||
| + | gacgtgatcggccaggtgcgccgccccgagatgggcgaccaggcccacatgccctacaccaccgccgtgatccacgaggt | ||
| + | gcagcgcttcggcgacatcgtgcccctgggcgtgacccacatgaccagccgcgacatcgaggtgcagggcttccgcatcc | ||
| + | ccaagggcaccaccctgatcaccaacctgagcagcgtgctgaaggacgagGTGAGCTTGCGGGGTTGCGAGCAACACTCC | ||
| + | AGCAACGAACAGTGCCCAAGTCAGGAATCTGCAGTCAGCCTGGGCTTTCGGCGGCTTTTTCTTGGGCAAACAGCTTGCAC | ||
| + | TCATGCCAGCGCGGCTTGTCCAGCCTCACTTGAGCTTTCCAGCTGCTACCAGCCGGGCTATACGACAGCGACAGAGCCAT | ||
| + | AGCGTGGAATCACTTATTTGGGTTGCCGAAGTAGCGGTCGGAGCGTGAGTTCTTGGTCAAGCCGCCCCTTATCCGGTTCC | ||
| + | TGTCCGTGTCTTTGTCCCTCGTTCACCCTTCGCGGCACCCTTCATCCCCTTGCTTGCAGgccgtgtgggagaagcccttc | ||
| + | cgcttccacccTgagcacttcctggacgcTcagggTcacttcgtgaagccTgaggccttcctgcccttcagcgcTggTcg | ||
| + | ccgcgcctgcctgggcgagcccctggcccgcatggagctgttcctgttcttcaccagcctgctgcagcacttcagcttca | ||
| + | gcgtggcTgcTggTcagccTcgccccagccacagccgcgtggtgagcttcctggtgaccccTagcccctacgagctgtgc | ||
| + | gccgtgccccgcgg | ||
Revision as of 12:26, 13 September 2023
CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 356
Illegal PstI site found at 1712
Illegal PstI site found at 2145 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 356
Illegal PstI site found at 1712
Illegal PstI site found at 2145
Illegal NotI site found at 862 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 356
Illegal PstI site found at 1712
Illegal PstI site found at 2145 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 356
Illegal PstI site found at 1712
Illegal PstI site found at 2145
Illegal NgoMIV site found at 999
Illegal NgoMIV site found at 1348 - 1000COMPATIBLE WITH RFC[1000]
ggcctggaggccctggtgcccctggccgtgatcgtggccatcttcctgctgctggtggacctgatgcaccgccgccagcg ctgggcTgcTcgctacagcccTggTcccctgccTctgcccgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGC TTGCAGATTTGACTTGCAACGCCCGCATTGTGTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAA CCCTGCGTCGCCGTTTCCATTTGCAGgcctgggcaacctgctgcacgtggacttccagaacaccccctactgcttcgacc agctgcgccgccgcttcggTgacgtgttcagcctgcagctggcTtggaccccTgtggtggtgctgaacggcctggcTgcT gtgcgcgaggccctggtgacccacggcgaggacaccgccgaccgcccTccTgtgcccatcacccagatcctgggTttcgg TccTcgcagccagggcgtgttcctggcccgctacggTccTgcTtggcgcgagcagcgccgcttcagcgtgagcaccctgc gcaacctgggcctgggcaagaagagcctggagcagtgggtgaccgaggaggccgcctgcctgtgcgccgccttcgccaac cacagcgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGTGTC GACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccgcccc ttccgcccTaacggTctgctggacaaggccgtgagcaacgtgatcgccagcctgacctgcggccgccgcttcgagtacga cgacccccgcttcctgcgcctgctggacctggcccaggagggcctgaaggaggagagcggcttcctgcgcgaggtgctga acgccgtgcccgtgctgctgcacatccccgccctggccggcaaggtgctgcgcttccagaaggccttcctgacccagctg gacgagctgctgaccgagcaccgcatgacctgggacccTgcTcagccTccTcgcgacctgaccgaggccttcctggcTga gatggagaagGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGT GTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccaa gggcaaccccgagagcagcttcaacgacgagaacctgcgcatcgtggtggccgacctgttcagcgccggcatggtgacca ccagcaccaccctggcctggggcctgctgctgatgatcctgcaccccgacgtgcagcgccgcgtgcagcaggagatcgac gacgtgatcggccaggtgcgccgccccgagatgggcgaccaggcccacatgccctacaccaccgccgtgatccacgaggt gcagcgcttcggcgacatcgtgcccctgggcgtgacccacatgaccagccgcgacatcgaggtgcagggcttccgcatcc ccaagggcaccaccctgatcaccaacctgagcagcgtgctgaaggacgagGTGAGCTTGCGGGGTTGCGAGCAACACTCC AGCAACGAACAGTGCCCAAGTCAGGAATCTGCAGTCAGCCTGGGCTTTCGGCGGCTTTTTCTTGGGCAAACAGCTTGCAC TCATGCCAGCGCGGCTTGTCCAGCCTCACTTGAGCTTTCCAGCTGCTACCAGCCGGGCTATACGACAGCGACAGAGCCAT AGCGTGGAATCACTTATTTGGGTTGCCGAAGTAGCGGTCGGAGCGTGAGTTCTTGGTCAAGCCGCCCCTTATCCGGTTCC TGTCCGTGTCTTTGTCCCTCGTTCACCCTTCGCGGCACCCTTCATCCCCTTGCTTGCAGgccgtgtgggagaagcccttc cgcttccacccTgagcacttcctggacgcTcagggTcacttcgtgaagccTgaggccttcctgcccttcagcgcTggTcg ccgcgcctgcctgggcgagcccctggcccgcatggagctgttcctgttcttcaccagcctgctgcagcacttcagcttca gcgtggcTgcTggTcagccTcgccccagccacagccgcgtggtgagcttcctggtgaccccTagcccctacgagctgtgc gccgtgccccgcgg
Design Notes
This part has been codon optimized for Chlamydomonas reinhardtii (https://www.kazusa.or.jp/codon/)
We inserted a few mutations to eliminate the recognition sites for our type IIS restriction enzymes BbsI and BsaI.
Source
https://www.uniprot.org/uniprotkb/P10635/entry