Difference between revisions of "Part:BBa K4806001"
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| − | Here are the links to the built constructs: | + | Here are the links to the built constructs:<br> |
<ul> | <ul> | ||
| − | <li>1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806205">BBa_K4806205</a>)</li> | + | <li>1. CYP2D6 gene with HA-tag for <i>Chlamydomonas reinhardtii</i> (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806205">BBa_K4806205</a>)</li> |
| − | <li>2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806207">BBa_K4806207</a>)</li> | + | <li>2. CYP2D6 gene with mStop for <i>Chlamydomonas reinhardtii</i> (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806207">BBa_K4806207</a>)</li> |
| − | <li>3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806206">BBa_K4806206</a>)</li> | + | <li>3. CYP2D6 gene with FLAG-tag for <i>Chlamydomonas reinhardtii</i> (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806206">BBa_K4806206</a>)</li> |
| − | <li>4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806208">BBa_K4806208</a>)</li> | + | <li>4. CYP2D6 gene for expression in the chloroplast for <i>Chlamydomonas reinhardtii</i> (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806208">BBa_K4806208</a>)</li> |
| − | <li>5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806215">BBa_K4806215</a>)</li> | + | <li>5. CYP2D6 tandem for expression together with the POR for <i>Chlamydomonas reinhardtii</i> (Phytobrick) (<a href=" https://parts.igem.org/Part:BBa_K4806215">BBa_K4806215</a>)</li> |
</ul> | </ul> | ||
</p> | </p> | ||
| − | + | <p> | |
| + | These constructs were transformed into <i>Chlamydomonas reinhardtii</i>. Besides the CYP2D6 coding sequence they contain an the paromomycin resistance cassette (<a href=" https://parts.igem.org/Part:BBa_K4806101 ">BBa_K4806101</a>), either the βSAP(i)-promotor (AβSAP(i) (<a href=" https://parts.igem.org/Part:BBa_K4806013">BBa_K4806013</a>) or the PSAD-promotor (<a href=" https://parts.igem.org/Part:BBa_K4806010">BBa_K4806010</a>), either the HA-tag (<a href=" https://parts.igem.org/Part:BBa_K4806215">BBa_K4806215</a>) or the FLAG-tag (<a href=" https://parts.igem.org/Part:BBa_K4806215">BBa_K4806215</a>) for detection and the tRPL23-terminator (<a href=" https://parts.igem.org/Part:BBa_K4806215">BBa_K4806215</a>). Additionally, one construct contains the CTPPSAD transit peptide to the chloroplast (<a href=" https://parts.igem.org/Part:BBa_K4806215">BBa_K4806215</a>) | ||
| + | </p> | ||
</html> | </html> | ||
Revision as of 10:52, 13 September 2023
CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)
This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (BBa_K4806013) and a terminator like tRPL23 (BBa_K3002006), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (BBa_K4806012) is recommended.
Constructs
We designed 5 level 2 parts containing CYP2D6 using the modular cloning system (MoClo).
Here are the links to the built constructs:
- 1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806205)
- 2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806207)
- 3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806206)
- 4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806208)
- 5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806215)
These constructs were transformed into Chlamydomonas reinhardtii. Besides the CYP2D6 coding sequence they contain an the paromomycin resistance cassette (BBa_K4806101), either the βSAP(i)-promotor (AβSAP(i) (BBa_K4806013) or the PSAD-promotor (BBa_K4806010), either the HA-tag (BBa_K4806215) or the FLAG-tag (BBa_K4806215) for detection and the tRPL23-terminator (BBa_K4806215). Additionally, one construct contains the CTPPSAD transit peptide to the chloroplast (BBa_K4806215)