Difference between revisions of "Part:BBa K4891001:Design"

 
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===Source===
 
===Source===
  
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There are four genes for converting PEP and E4P into shikimate, including aroG (encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase), aroB (encoding 3-dehydroquinate synthase), aroD (encoding 3-dehydroquinate dehydratase), and aroE (encoding shikimate dehydrogenase), and these genes originate from Escherichia coli.
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This gene is originated from Escherichia coli MG1655.
  
 
===References===
 
===References===

Revision as of 09:23, 13 September 2023


aroB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 906
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For one thing, the usage of AroGfbr could alleviate the feedback inhibition of aromatic amino acids. For another thing, AroB optimization enable to enhance expression level. As a result, shikimate biosynthesis is improved owing to the enhanced metabolic flow.


Source

This gene is originated from Escherichia coli MG1655.

References