Difference between revisions of "Part:BBa K4806001"
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<p>This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (<a href=” https://parts.igem.org/ Bba_K4806013”>Bba_K4806013</a>) and a terminator like tRPL23 (<a href=”https://parts.igem.org/Part:BBa_K3002006”>BBa_K3002006</a>), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (<a href=” https://parts.igem.org/ Bba_K4806012”>Bba_K4806012</a>) is recommended. </p> | <p>This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (<a href=” https://parts.igem.org/ Bba_K4806013”>Bba_K4806013</a>) and a terminator like tRPL23 (<a href=”https://parts.igem.org/Part:BBa_K3002006”>BBa_K3002006</a>), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (<a href=” https://parts.igem.org/ Bba_K4806012”>Bba_K4806012</a>) is recommended. </p> | ||
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<h2><u>Constructs</u></h2> | <h2><u>Constructs</u></h2> | ||
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Revision as of 09:08, 13 September 2023
CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)
This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (Bba_K4806013) and a terminator like tRPL23 (BBa_K3002006), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (Bba_K4806012) is recommended.
Constructs
Fig.1 Construct design
We designed 5 level 2 parts containing CYP2D6 using the modular cloning system (MoClo).
Here are the links to the built constructs: and transformed these into Chlamydomonas reinhardtii.
- 1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) ()
- 2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) ()
- 3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) ()
- 4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) ()
- 5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) ()