Difference between revisions of "Part:BBa K4806001"
Line 8: | Line 8: | ||
<img src=”https://static.igem.wiki/teams/4806/wiki/registry/registry-test.png”/> | <img src=”https://static.igem.wiki/teams/4806/wiki/registry/registry-test.png”/> | ||
</p> | </p> | ||
+ | <ul> | ||
+ | <li>1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) ()</li> | ||
+ | <li>2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) ()</li> | ||
+ | <li>3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) ()</li> | ||
+ | <li>4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) ()</li> | ||
+ | <li>5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) ()</li> | ||
+ | </ul> | ||
+ | |||
</html> | </html> |
Revision as of 09:06, 13 September 2023
CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)
This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (Bba_K4806013) and a terminator like tRPL23 (BBa_K3002006), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (Bba_K4806012) is recommended.
Constructs
- 1. CYP2D6 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) ()
- 2. CYP2D6 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) ()
- 3. CYP2D6 gene with FLAG-tag for Chlamydomonas reinhardtii (Phytobrick) ()
- 4. CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick) ()
- 5. CYP2D6 tandem for expression together with the POR for Chlamydomonas reinhardtii (Phytobrick) ()