Difference between revisions of "Part:BBa K4806013"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4806013 short</partinfo> | <partinfo>BBa_K4806013 short</partinfo> | ||
| + | <!DOCTYPE html> | ||
<html> | <html> | ||
| + | <body> | ||
<p>This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (<a href=” https://parts.igem.org/Part:BBa_K4806013”>BBa_K4806013</a>) and a terminator like tRPL23 (<a href=” https://parts.igem.org/Part:BBa_K4806008”>BBa_K4806008</a>), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (<a href=” https://parts.igem.org/Part:BBa_K4806012”>BBa_K4806012</a>) is recommended. </p> | <p>This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (<a href=” https://parts.igem.org/Part:BBa_K4806013”>BBa_K4806013</a>) and a terminator like tRPL23 (<a href=” https://parts.igem.org/Part:BBa_K4806008”>BBa_K4806008</a>), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (<a href=” https://parts.igem.org/Part:BBa_K4806012”>BBa_K4806012</a>) is recommended. </p> | ||
<h2><u>Constructs</u></h2> | <h2><u>Constructs</u></h2> | ||
| + | </body> | ||
</html> | </html> | ||
Revision as of 07:39, 13 September 2023
AβSAP(i)-promotor for Chlamydomonas reinhardtii (Phytobrick)
<!DOCTYPE html>
This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (BBa_K4806013) and a terminator like tRPL23 (BBa_K4806008), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (BBa_K4806012) is recommended.
Constructs
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 530
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 268
- 1000COMPATIBLE WITH RFC[1000]